Protein Extraction
  • DE301-01.JPG
DE301-01.JPG

ProteinExt® Mammalian Membrane Protein Extraction Kit


Product Details

ProteinExt® Mammalian Membrane Extraction Kit provides a simple and efficient method to extract membrane proteins from mammalian cells and tissues. Native proteins can be obtained within 70 minutes without ultracentrifugation. Up to 90% efficiency for membrane proteins have at least 1-2 transmembrane domains. The extracted protein is suitable for a variety of downstream applications, including SDS-PAGE, Western Blot, ELISA, and enzyme-activity assays.

Storage
ProteinExtTM Protease Inhibitor Cocktail, EDTA-free (100×) at -20℃ for one year; Others at 2-8℃ for one year.

Shipping
ProteinExtTM Protease Inhibitor Cocktail, EDTA-free (100×) on dry ice (-70℃); Others on ice bag (4℃). 

Product Contents


ComponentDE301-01 (50 rxns)
Membrane Protein Extraction Buffer I (MPEB I)50 ml
Membrane Protein Extraction Buffer II (MPEB II)7.5 ml
Membrane Protein Extraction Buffer III (MPEB III)15 ml
ProteinSafeTM Protease Inhibitor Cocktail, EDTA-free (100×)1 ml


Citations

Guo M, Zhang C , Wang Y , et al. Progesterone Receptor Membrane Component 1 Mediates Progesterone-Induced Suppression of Oocyte Meiotic Prophase I and Primordial Folliculogenesis[J]. Scientific Reports, 2016, 16 (6):36869. 

Fang W, Fa ZZ, Xie Q , et al. Complex Roles of Annexin A2 in Host Blood-Brain Barrier Invasion by Cryptococcus neoformans[J]. CNS Neuroscience & Therapeutics, 2017, 23(4):291-300.

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