TransGen Biotech has launched EasyPure® Fast Cell RNA Kit. This kit uses a unique lysis solution to rapidly lyse animal cells, simultaneously inactivate endogenous RNase in cells. It can quickly (<7 minutes) complete the extraction of RNA from 1-6×106 animal cells, without phenol and chloroform. Purified total RNA with high purity can be used for RT-PCR, qRT-PCR, chip analysis, Northern Blot, NGS, etc.
EasyPure ® Fast Cell RNA Kit (ER111)
Features
• Simple operation: operate at room temperature during the whole process, no need for low-temperature centrifugation
• Fast: extraction completes in 7 minutes
• Safty: no need for organic reagents such as phenol and chloroform
• High purity: efficient removal of impurity contamination
Experiment Process
![1704950349150661.png 图1调整后.png](/data/upload/image/20240111/1704950349150661.png)
Experiment Data
High yield
Extract RNA from Hela, 293T, HEK293, A549, Jurkat, CHO, and hybridoma cells by using products from TransGen and Company S respectively. NanoDrop detects RNA concentration, and gel electrophoresis detects RNA quality. The results show that the TransGen product extracts RNA with high concentration and good results.
![1704952140397280.png 表1.png](/data/upload/image/20240111/1704952140397280.png)
Hela
![1704950929343580.png 图2调整.png](/data/upload/image/20240111/1704950929343580.png)
293T
![1704951098987235.png 图3调整.png](/data/upload/image/20240111/1704951098987235.png)
HEK293
![1704951235111916.png 图4调整.png](/data/upload/image/20240111/1704951235111916.png)
CHO
![1704951335995601.png 图5调整.png](/data/upload/image/20240111/1704951335995601.png)
Hybridoma Cells
![1704951418283438.png 图6调整.png](/data/upload/image/20240111/1704951418283438.png)
A549
![1704951519560694.png 图7调整.png](/data/upload/image/20240111/1704951519560694.png)
Jurkat
![1704951615192080.png 图8调整.png](/data/upload/image/20240111/1704951615192080.png)
No inhibition of downstream qPCR reactions
Extract RNA from Hela, 293T and hybridoma cells by using products from TransGen and Company S, respectively. 200 ng of RNA was used as a template for reverse transcription and qPCR detection. The results showed that the RNA extracted by TransGen products did not inhibit the downstream qPCR reaction.
![1704951738184288.png 图9.png](/data/upload/image/20240111/1704951738184288.png)
![1704952032480616.png 表2调整.png](/data/upload/image/20240111/1704952032480616.png)