Plasmid DNA Purification
  • EM101-01.JPG

EasyPure® Plasmid MiniPrep Kit

Product Details

EasyPure® Plasmid MiniPrep Kit uses a modified alkaline lysis method to isolate high-quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation and DNA sequencing.

• Simple and fast: the whole procedure can be performed in 20 minutes.
• High yield: DNA yield up to 40 μg.

at room temperature (15-25°C) in a dry place for one year. 

 at room temperature.

Product Contents

ComponentEM101-01 (50 rxns)EM101-02 (200 rxns)
Resuspension Buffer (RB)15 ml60 ml
Lysis Buffer (LB)15 ml60 ml
Neutralization Buffer (NB)20 ml80 ml
Wash Buffer (WB)10 ml2×20 ml
Elution Buffer (EB)5 ml10 ml
RNase A (10 mg/ml)150 μl600 μl
Mini-Plasmid Spin Column with Collection Tubes50 each2×100 each


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Li X K , Chu Z R , Liu Y J , et al. Molecular characterization of microbial populations in full-scale biofilters treating iron, manganese and ammonia containing groundwater in Harbin, China[J]. Bioresource Technology, 2013, 147(Complete):234-239.

Wang B , Xiao J H , Bian S N , et al. Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi[J]. PLOS ONE, 2013, 8(1):e53907.

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