Plasmid DNA Purification
  • EM111-01.JPG

EasyPure® HiPure Plasmid MiniPrep Kit

Product Details

EasyPure® HiPure Plasmid MiniPrep Kit provides an efficient way to isolate high yield (up to 40 μg) and high quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization.Endotoxin is removed by a simple incubation on column with a novel buffer. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.

• Fast: the whole procedure can be performed in 20 minutes.
• Simple: endotoxin is removed on column.
• High yield: DNA yield up to 40 μg.

at room temperature (15-25°C) in a dry place for one year.

at room temperature.

Product Contents

ComponentEM111-01 (50 rxns)
Resuspension Buffer (RB)15 ml
Lysis Buffer (LB)15 ml
Neutralization Buffer (NB)20 ml
ToxinOut Buffer (TB)15 ml
Wash Buffer (WB)10 ml
Elution Buffer (EB)5 ml
RNase A (10 mg/ml)150 μl
Mini-Plasmid Spin Columns with Collection Tubes50 each


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