Products

Plasmid DNA Purification
  • EM122.jpg
EM122.jpg

EasyPure® EndoFree Plasmid MaxiPrep Kit


Product Details

EasyPure® EndoFree Plasmid MaxiPrep Kit uses alkaline lysis method to extract plasmid DNA from no more than 500 ml of LB E.

coli culture. Unique silica membrane adsorption technique to efficiently bind plasmid DNA. The solution contains indicators that

can indicate whether the lysis and neutralization are complete through the change of color, so as to visualize the procedure.

Endotoxin is removed on the column, which is fast and simple to use. The purified DNA is suitable for a variety of molecular

biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.


Features

• Visualized operation: solution LB (blue) indicates whether the lysis and neutralization are complete through the change of color, so

as to ensure the quality of plasmid extraction.

• Fast: the whole procedure can be performed within one hour.

• Easy to use: on-column rapid endotoxin removal.

• High yield (up to 4 mg nucleic acid load in the purification column).


Storage
at room temperature (15-25 ℃) under dry conditions for one year.


Shipping
at room temperature.

Product Contents


ComponentEM122-01(10 rxns)
Resuspension Buffer (RB)120 ml
Lysis Buffer (LB, Blue)120 ml
Neutralization Buffer 1 (NB1)120 ml
Activation Buffer (AB)55 ml
Wash Buffer (WB)25 ml
Elution Buffer (EB)25 ml
RNase A (10 mg/ml )1.2 ml
Maxi-Plasmid Spin Column with Collection Tube10 each
Push Filter10 each
50 ml Collection Tube10 each


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