EasyPure® EndoFree Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA from≤500 ml(LB) of bacterial culture. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the operation. Plasmid purification by the endotoxin removal solution. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection. The kit can be used 10 times based on a single 100 ml to 200 ml bacterial input.
Features
• Operational visualization: solution LB (blue) indicates whether the lysis and neutralization are complete through the
change of color.
• Fast: the whole procedure can be performed in one hour.
• High purity: high-purity, endotoxin-free (<0.1EU/μg) transfection-grade plasmid DNA can be prepared.
• High yield (up to 4 mg nucleic acid load in the purification column).
Storage
at room temperature (15-25 ℃) under dry conditions for one year.
Shipping
at room temperature.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
