Plasmid DNA Purification
  • EM121-01.JPG

EasyPure® EndoFree Plasmid MaxiPrep Kit

Product Details

EasyPure® EndoFree Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA from≤500 ml(LB) of bacterial culture. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the operation. Plasmid purification by the endotoxin removal solution. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection. The kit can be used 10 times based on a single 100 ml to 200 ml bacterial input.


• Operational visualization: solution LB (blue) indicates whether the lysis and neutralization are complete through the

change of color.

• Fast: the whole procedure can be performed in one hour.

• High purity: high-purity, endotoxin-free (<0.1EU/μg) transfection-grade plasmid DNA can be prepared.

• High yield (up to 4 mg nucleic acid load in the purification column).

at room temperature (15-25 ℃) under dry conditions for one year.

at room temperature.

Product Contents

ComponentEM123-01(10 rxns)
Resuspension Buffer (RB)120 ml
Lysis Buffer (LB, Blue)120 ml
Neutralization Buffer 1 (NB1)120 ml
Activation Buffer (AB)55 ml
Endotoxin Removal Buffer (ER)33 ml
Wash Buffer (WB)25 ml
Elution Buffer (EB)25 ml
RNase A (10 mg/ml )1.2 ml
Maxi-Plasmid Spin Column with Collection Tube10
Push Filter10
50 ml Collection Tube10

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