Products

Plasmid DNA Purification
  • EM123.jpg
EM123.jpg

EasyPure® EndoFree Pro Plasmid MaxiPrep Kit


Product Details

EasyPure® EndoFree Pro Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA

from≤500 ml(LB) of bacterial culture. The solution contains indicators that can indicate whether the lysis and

neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the

operation. Plasmid purification process include special endotoxin removal solution. Endotoxin level can reach as low as

<0.1 EU/μg. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme

digestion, ligation, transformation, DNA sequencing, and transfection.


Features

• Visualized operation: solution LB (blue) indicates whether the lysis and neutralization are complete through the

change of color.

• Fast: the whole procedure can be performed in one hour.

• High purity: high-purity, endotoxin-free (<0.1EU/μg) transfection-grade plasmid DNA can be prepared.

• High yield (up to 4 mg nucleic acid load in the purification column).


Product Contents


ComponentEM123-01(10 rxns)
Resuspension Buffer (RB)120 ml
Lysis Buffer (LB, Blue)120 ml
Neutralization Buffer 1 (NB1)120 ml
Activation Buffer (AB)55 ml
Endotoxin Removal Buffer (ER)33 ml
Wash Buffer (WB)25 ml
Elution Buffer (EB)25 ml
RNase A (10 mg/ml )1.2 ml
Maxi-Plasmid Spin Column with Collection Tube10 each
Push Filter10 each
50 ml Collection Tube10 each


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