PCR Enzymes
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TransTaq® DNA Polymerase High Fidelity(HiFi)

Product Details

TransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.

• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR

at -20 ℃ for two years

Dry ice (-70 ℃)

Product Contents

TransTaq® HiFi DNA Polymerase250 U×1500 U×1500 U×6
10×TransTaq® HiFi Buffer I1.2 ml×11.2 ml×11.2 ml×6
10×TransTaq® HiFi Buffer II1.2 ml×11.2 ml×11.2 ml×6
2.5 mM dNTP-/400 μl×1-/800 μl×1-/800 μl×6
10×GC Enhancer200 μl×1400 μl×11 ml×1
6×DNA Loading Buffer500 μl×11 ml×11 ml×2


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