PCR Enzymes
  • AP151-01.JPG
AP151-01.JPG

TransStart® TopTaq DNA Polymerase


Product Details

TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

• Compared with TransStart® Taq DNA Polymerase, TransStart®TopTaq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.
TransStart® TopTaq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• The specificity is higher than antibody based or chemically modified hot start DNA polymerases.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.

Applications
• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP151-01/11AP151-02/12AP151-03/13
TransStart® TopTaq DNA Polymerase250 U×1500 U×1500 U×6
10×TransStart® TopTaq Buffer1.2 ml1.2 ml×21.2 ml×12
2.5 mM dNTPs-/800 μl×1-/800 μl×2-/1.2 ml×8
10×GC Enhancer200 μl×1400 μl×11 ml×1
6×DNA Loading Buffer500 μl×11 ml×11 ml×2


Citations

Su Y , Li B , Zhu W Y . Fecal microbiota of piglets prefer utilizing dl-lactate mixture as compared to d-lactate and l-lactate in vitro[J]. Anaerobe, 2013, 19(Complete):27-33.

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