PCR Enzymes
  • AP141-01.JPG

TransStart® Taq DNA Polymerase

Product Details

TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins. At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

TransStart® Taq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.

• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR

at -20 ℃ for two years

Dry ice (-70 ℃)

Product Contents

TransStart® Taq DNA Polymerase250 U×1500 U×1500 U×6
10×TransStart® Taq Buffer1.2 ml1.2 ml×21.2 ml×12
2.5 mM dNTPs-/800 μl×1-/800 μl×2-/1.2 ml×8
10×GC Enhancer200 μl×1400 μl×11 ml×1
6×DNA Loading Buffer500 μl×11 ml×11 ml×2


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