PCR Enzymes
  • AP301-01.JPG
AP301-01.JPG

TransStart® KD Plus DNA Polymerase


Product Details

TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.

• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP301-01/11AP301-02/12AP301-03/13
TransStart® KD Plus DNA Polymerase100 U×1200 U×1200 U×6
TransStart® KD Plus Buffer1.2 ml×11.2 ml ×21.2 ml ×12
2.5 mM dNTPs- / 500 μl×1- / 1 ml ×1- / 1 ml ×6
50 mM MgSO4200 μl×1400 μl ×11 ml ×1
6×DNA Loading Buffer500 μl×11 ml ×11 ml ×2


Citations

Li P, Du J, Goodier JL, et al.  Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic Acids Res. 2017 May 5;45(8):4619-4631. 

Zhao K , Du J , Rui Y , et al. Evolutionarily conserved pressure for the existence of distinct G2/M cell cycle arrest and A3H inactivation functions in HIV-1 Vif[J]. Cell Cycle, 2015, 14(6):10.

Xie M , Na‐Na Ren, Yan‐Chun You, et al. Molecular characterisation of two α‐esterase genes involving chlorpyrifos detoxification in the diamondback moth, Plutella xylostella[J]. Pest Management Science, 2017, 73(6):1204.

Wang X , Liu J , Zhu Z , et al. The heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5 controls Caenorhabditis elegans Q neuroblast polarization during migration[J]. Developmental Biology, 2015, 399(2):306-314.

Xie M , Na‐Na Ren, Yan‐Chun You, et al. Molecular characterisation of two α‐esterase genes involving chlorpyrifos detoxification in the diamondback moth, Plutella xylostella[J]. Pest Management Science, 2017, 73(6):1204.


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