PCR Enzymes
  • AP101-01.JPG

TransFast® Taq DNA Polymerase

Product Details

TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.

• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

• Routine PCR
• High throughput PCR
• Colony PCR

at -20 ℃ for two years

Dry ice (-70 ℃)

Product Contents

TransFast® Taq DNA Polymerase500 U×1500 U×6
10×TransFast® Taq Buffer1.2 ml ×11.2 ml ×6
2.5 mM dNTPs- / 800 μl ×1- / 800 μl ×6
6×DNA Loading Buffer1 ml×11 ml ×2

Experimental Data



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Zhu X J , Sun S , Xie B , et al. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769.

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