PCR Enzymes
  • AP101-01.JPG
AP101-01.JPG

TransFast® Taq DNA Polymerase


Product Details

TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.

• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

Applications
• Routine PCR
• High throughput PCR
• Colony PCR

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP101-01/11AP101-02/12
TransFast® Taq DNA Polymerase500 U×1500 U×6
10×TransFast® Taq Buffer1.2 ml ×11.2 ml ×6
2.5 mM dNTPs- / 800 μl ×1- / 800 μl ×6
6×DNA Loading Buffer1 ml×11 ml ×2


Experimental Data

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Citations

Cao Y X , Xiao W H , Liu D , et al. Biosynthesis of odd-chain fatty alcohols in Escherichia coli[J]. Metabolic Engineering, 2015, 29:113-123. 

Wang Y , Hou Y , Gu H , et al. The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis[J]. The Plant Journal, 2012, 69(2):227-240.                                                                                    

Zhang Y , Ji G , Wang R . Drivers of nitrous oxide accumulation in denitrification biofilters with low carbon:nitrogen ratios[J]. Water Research, 2016,106:79-85.

Zhu X J , Sun S , Xie B , et al. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769.

Xia W, Lu H, Xia M, et al. A Novel Glycoside Hydrolase Family 113 Endo-β-1,4-Mannanase from Alicyclobacillus sp. Strain A4 and Insight into the Substrate Recognition and Catalytic Mechanism of This Family.[J]. Applied & Environmental Microbiology, 2016, 82(9):2718.

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