PCR Enzymes
  • AP112-01.JPG
AP112-01.JPG

EasyTaq® DNA Polymerase for PAGE


Product Details

EasyTaq® DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.

• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 3 kb.

Applications
Short fragment PCR

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP112-01/11AP112-02/12
EasyTaq® DNA Polymerase for PAGE2500 U2500 U×4
10×EasyTaq® Buffer for PAGE1.2 ml ×51.2 ml×20
2.5 mM dNTPs- / 800 μl×5- / 800 μl×20
6×DNA Loading Buffer1 ml1 ml ×4


Citations

Huang YS , Li ZB , Ning YF , et al. Isolation and characterization of microsatellite loci in hybrid giant tiger grouper[J]. Genetics & Molecular Research Gmr, 2015, 14(4):14706.

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