PCR Enzymes
  • AP111-01.JPG
AP111-01.JPG

EasyTaq® DNA Polymerase


Product Details

EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.

• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

Applications
• Routine PCR
• High throughput PCR
• Colony PCR

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents

Without dNTPs

ComponentAP111-01AP111-02AP111-03AP111-04
EasyTaq® DNA Polymerase500 U×1500 U×62500 U×45000 U×10
10×EasyTaq® Buffer1.2 ml×11.2 ml×61.2 ml×201.2 ml×100
6×DNA Loading Buffer1 ml×11 ml×21 ml×41 ml×20

With dNTPs

ComponentAP111-11AP111-12AP111-13
EasyTaq® DNA Polymerase500 U×1500 U×62500 U×4
10×EasyTaq® Buffer1.2 ml×11.2 ml×61.2 ml×20
2.5 mM dNTPs800 μl×1800 μl ×6800 μl×20
6×DNA Loading Buffer1 ml×11 ml×21 ml×4


Citations

Zhu C , Gao Y , Guo H , et al. Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution[J]. Cell Stem Cell, 2017, 20(5):720-731.

Shao N , Jiang S , Zhang M , et al. MACRO: A Combined Microchip-PCR and Microarray System for High-throughput Monitoring of Genetically Modified Organisms[J]. Analytical Chemistry, 2013, 86(2):1269-1276.

Li J , Fu J , Chen Y , et al. The U6, Biogenesis-Like 1, Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3′ End Processing of U6 snRNA[J]. Molecular Plant, 2017, 10(3):470-482.

Simond AM , Rao T , Zuo D , et al. ErbB2-positive mammary tumors can escape PI3K-p110α loss through downregulation of the Pten tumor suppressor[J]. Oncogene, 2017, 36(43):6059-6066.

Li Y , Guo SJ , Shao N , et al. A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray[J]. LAB ON A CHIP, 2011, 11(21):3609-8.

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