PCR Enzymes
  • AP111-01.JPG

EasyTaq® DNA Polymerase

Product Details

EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.

• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

• Routine PCR
• High throughput PCR
• Colony PCR

at -20 ℃ for two years

Dry ice (-70 ℃)

Product Contents

Without dNTPs

EasyTaq® DNA Polymerase500 U×1500 U×62500 U×45000 U×10
10×EasyTaq® Buffer1.2 ml×11.2 ml×61.2 ml×201.2 ml×100
6×DNA Loading Buffer1 ml×11 ml×21 ml×41 ml×20

With dNTPs

EasyTaq® DNA Polymerase500 U×1500 U×62500 U×4
10×EasyTaq® Buffer1.2 ml×11.2 ml×61.2 ml×20
2.5 mM dNTPs800 μl×1800 μl ×6800 μl×20
6×DNA Loading Buffer1 ml×11 ml×21 ml×4


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