Low temperature Uracil-DNA Glycosylase (UDG) is a recombinant protein derived from psychrophile marine bacteria that have been modified to be expressed in Escherichia coli. UDG enzyme can effectively hydrolyze the N-glycosidic bond between the uracil (dU) base and deoxyribose in single or double-stranded DNA, thus releasing free uracil and producing pyrimidine deficiency sites, which are mainly used to eliminate the aerosol contamination caused by PCR products containing dU. This product is easily hydrolyzed and fractured at high temperatures or pH. Compared with conventional UDG enzymes, the product can operate at a lower temperature (20°C), i.e., when preparing qPCR or qRT-PCR reaction systems at room temperature, it can degrade contaminated dU-containing templates. This enzyme does not degrade dU-containing cDNA (DNA/RNA hybrid form) produced during reverse transcription. This product has high activity in most PCR reaction buffers and is suitable for PCR/qPCR, RT-PCR/qRT-PCR, and LAMP/RT-LAMP.
Applications
Remove uracil base from single-stranded or double-stranded DNA; prevent aerosol contamination of PCR products containing dU and improve the specificity of the reaction.
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
