Modifying Enzymes
  • LH101-01.JPG
LH101-01.JPG

RNase H


Product Details

Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA. This product is purified from E. coli expressing the recombinant rnhA gene on a plasmid with 17 kDa molecular weight and it can be inactivated by heating at 65°C for 20 minutes.

Definition of Activity Unit
One unit is defined as the amount of RNase H that solubilizes 1 nmol RNA in the 3 tagged poly(rA)·poly(dT) hybrid chain in 20 minutes at 37°C in 50 μl system.

Applications
Removal of mRNA prior to synthesis of second strand cDNA.
Identification of RNA-DNA Hybrid.

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents

ComponentLH101-01LH101-02
RNase H100 units5×100 units
10×RNase H Buffer1 ml5×1 ml

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