The mammalian CD3 molecule, which is expressed on the surface of mature T lymphocytes, is an important surface marker of T cell population and mainly consists of δ, ε, γ and ζ chains. CD3 molecules are non-covalent bonded to T-cell receptor (TCR) to form a TCR-CD3 complex. When antigen presenting cells (APCs) activate TCR, TCR-mediated signals are transmitted to the cell interior via CD3δ, CD3ε, CD3γ or CD3ζ. All cytoplasmic regions of CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs), these motifs are phosphorylated by the Src family protein tyrosine kinases LCK and FYN once bound to TCR, thereby activating downstream signaling pathways[1]. In addition to its signal transduction role in T cell activation, CD3ε also plays a critical role in the proper development of T cells. The assembly of TCR-CD3 complex is initiated by the formation of two heterodimers, CD3δ/CD3ε and CD3γ/CD3ε, and the special sequence of CD3ε cytoplasmic region can contribute to TCR-CD3 complex internalization, down-regulating the expression of TCR on the cell surface[2, 3].This product is the mouse anti-human CD3 monoclonal antibody, which is used for the specific detection of human CD3 by FC.
Applicable Experiments and Dilution
· FC: 1:100 dilution is recommended.
Storage
PBS (pH7.4), 0.05% ProClin 300, 50% Glycerol; at -20℃ for two years, avoid repeated freeze-thawing.
Shipping
Dry ice (-70℃)
[1] Barber EK, Dasgupta JD, Schlossman SF, et al. The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex [J]. Proc Natl Acad Sci U S A. 1989, 86(9): 3277-81.
[2] Borroto A, Lama J, Niedergang F, et al. The CD3 epsilon subunit of the TCR contains endocytosis signals [J]. J Immunol. 1999, 163(1): 25-31.
[3] Martin-Blanco N, Jiménez Teja D, Bretones G, et al. CD3ε recruits Numb to promote TCR degradation [J]. International Immunology. 2016, 28(3): 127-37.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
