Genomic DNA Purification
  • EE101-01.JPG
EE101-01.JPG

EasyPure® Genomic DNA Kit


Product Details

EasyPure® Genomic DNA Kit provides a simple and convenient way to efficiently isolate high-quality genomic DNA from a variety of materials (mammalian cells, tissues, mouse tails, E. coli and yeast). Cells and tissues are enzymatically lysed. DNA is specifically bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot, etc.

• Fast extraction, high yield (up to 15 μg).

• High integrity of cDNA ensured by mild lysis conditions without physical disruption, thus reducing damage to gDNA during cell lysis process.

• High purity enabled by spin column which can efficiently and specifically bind to DNA and removs protein, salts, lipids or other contaminants.


Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) in a dry place for one year.


Shipping
RNase A on dry ice (-70 °C); Others at room temperature.

Product Contents


ComponentEE101-01 (50 rxns)EE101-02 (200 rxns)EE101-11 (50 rxns)EE101-12 (200 rxns)
Lysis Buffer 2 (LB2)6 ml24 ml6 ml24 ml
Binding Buffer 2 (BB2)28 ml110 ml28 ml110 ml
Clean Buffer 2 (CB2)15 ml60 ml15 ml60 ml
Wash Buffer 2 (WB2)12 ml2×22 ml12 ml2×22 ml
Elution Buffer (EB)25 ml80 ml25 ml80 ml
RNase A (20 mg/ml)1 ml4×1 ml00
Proteinase K (20 mg/ml)1 ml4×1 ml1 ml4×1 ml
Genomic Spin Column with Collection Tubes50 each200 each50 each200 each


Citations

Characterization of a novel strain phylogenetically related to Kocuria rhizophila and its chemical modification to improve performance of microbial fuel cells[J]. Biosensors and Bioelectronics, 2015, 69:113-120.

Xu X , Wang S , Liu J , et al. Hypoxia induces downregulation of soluble guanylyl cyclase β1 by miR-34c-5p[J]. Journal of Cell Science, 2012, 125(24):6117-6126.

Geng J , Wang X , Wei H , et al. Efficient Attenuation of NK Cell-Mediated Liver Injury through Genetically Manipulating Multiple Immunogenes by Using a Liver-Directed Vector[J]. The Journal of Immunology, 2013, 190(9):4821-4829. 

Yao P , Chen X , Yan Y , et al. Glutaredoxin 1, glutaredoxin 2, thioredoxin 1, and thioredoxin peroxidase 3 play important roles in antioxidant defense in Apis cerana cerana[J]. Free Radical Biology and Medicine, 2014, 68:335-346.

Luo J , Yang J , He H , et al. A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs[J]. Bioresource Technology, 2013, 139:141-148.


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