Genomic DNA Purification
  • EE121-01.JPG
EE121-01.JPG

EasyPure® Blood Genomic DNA Kit


Product Details

EasyPure® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood. Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column.

• Simple and fast, red cell lysis buffer is no longer needed.
• Complete removal of contaminants and inhibitors.
• DNA yield up to 40 μg.
• Column based purification, no organic extraction or ethanol precipitation.
• Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μl.

Applications
PCR, restriction enzyme digestion and Southern blot

Storage
At room temperature (15-25°C) in a dry place for one year; RNase A at -20°C for two years.

Shipping
RNase A on dry ice (-70 °C); others at room temperature.

Product Contents


ComponentEE121-01 (50 rxns)EE121-02(200 rxns)EE121-11 (50 rxns)EE121-12(200 rxns)
Binding Buffer 3 (BB3)30 ml110 ml30 ml110 ml
Clean Buffer 3 (CB3)6 ml24 ml6 ml24 ml
Wash Buffer 3 (WB3)12 ml2×22 ml12 ml2×22 ml
Elution Buffer (EB)25 ml80 ml25 ml80 ml
RNase A (20 mg/ml)500 μl2×1 ml00
Proteinase K (20 mg/ml)1 ml4×1 ml1 ml4×1 ml
Genomic Spin Columns with Collection Tubes50 each200 each50 each200 each


Citations

Li X , Zhu Y , Liu P , et al. Assessment of genetic diversity in Chinese eared pheasant using fluorescent-AFLP markers.[J]. Molecular Phylogenetics & Evolution, 2010, 57(1):429-433.

Zhang S , Wang Q M , Ding X P , et al. Association of polymorphisms in PATE1 gene with idiopathic asthenozoospermia in Sichuan, China[J]. Journal of Reproductive Immunology, 2016, 118(2):54-60.

Ablimit A , Qin W , Shan W , et al. Genetic diversities of cytochrome B in Xinjiang Uyghur unveiled its origin and migration history[J]. BMC Genetics, 2013, 14(1):100.

Xin C , Hao Y , Tong-Wen O , et al. Genetic Variations in Inflammatory Response Genes and Their Association with the Risk of Prostate Cancer[J]. BioMed Research International, 2015, 2015:1-7.

Lu Z H , Gu X J , Shi K Z , et al. Association between genetic polymorphisms of inflammatory response genes and the risk of ovarian cancer[J]. Journal of the Formosan Medical Association, 2016, 115(1):31-7.


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