Genomic DNA Purification
  • EE121-01.JPG

EasyPure® Blood Genomic DNA Kit

Product Details

EasyPure® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood. Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column.

• Simple and fast, red cell lysis buffer is no longer needed.
• Complete removal of contaminants and inhibitors.
• DNA yield up to 40 μg.
• Column based purification, no organic extraction or ethanol precipitation.
• Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μl.

PCR, restriction enzyme digestion and Southern blot

At room temperature (15-25°C) in a dry place for one year; RNase A at -20°C for two years.

RNase A on dry ice (-70 °C); others at room temperature.

Product Contents

ComponentEE121-01 (50 rxns)EE121-02(200 rxns)EE121-11 (50 rxns)EE121-12(200 rxns)
Binding Buffer 3 (BB3)30 ml110 ml30 ml110 ml
Clean Buffer 3 (CB3)6 ml24 ml6 ml24 ml
Wash Buffer 3 (WB3)12 ml2×22 ml12 ml2×22 ml
Elution Buffer (EB)25 ml80 ml25 ml80 ml
RNase A (20 mg/ml)500 μl2×1 ml00
Proteinase K (20 mg/ml)1 ml4×1 ml1 ml4×1 ml
Genomic Spin Columns with Collection Tubes50 each200 each50 each200 each


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