EasyPure® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells. Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.
• Fast: the whole process can be completed in 50 minutes.
• High yield: DNA yield up to 20 μg.
Applications
PCR, restriction enzyme digestion and Southern blot
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature.
Liu Y, Chen Z, Jiang Z, et al. Biochemical characterization of a novel L-Asparaginase from Paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes[J]. International Journal of Biological Macromolecules,2017, 104(Pt A):1055-1063.
Shi R , Liu Y , Mu Q , et al. Biochemical characterization of a novel L-asparaginase from Paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes[J]. International Journal of Biological Macromolecules, 2017, 96:93-99.
Zheng X , Liu H , Song L , et al. Phaeocystidibacter marisrubri sp. nov. a member of the family Cryomorphaceae isolated from Red Sea sediment[J]. International Journal of Systematic and Evolutionary Microbiology, 2015, 65(7):2199.
Duan D , Cheng T . Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high‐throughput sequencing[J]. Journal of Basic Microbiology, 2016, 57(4):302.
Cheng T Y , Liu G H . PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks[J]. Acta Parasitologica, 2017, 62(2):269-272.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
