Genomic DNA Purification
  • EE161-01.JPG
EE161-01.JPG

EasyPure® Bacteria Genomic DNA Kit


Product Details

EasyPure® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells. Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.

• Fast: the whole process can be completed in 50 minutes.
• High yield: DNA yield up to 20 μg.

Applications
PCR, restriction enzyme digestion and Southern blot

Storage
RNase A at -20 for two years; others at room temperature (15-25) for one year.

Shipping

RNase A on dry ice (-70℃); others at room temperature.

Product Contents


ComponentEE161-01 (50 rxns)EE161-11 (50 rxns)
Resuspension Buffer11 (RB11)12 ml12 ml
Lysis Buffer11 (LB11)6 ml6 ml
Binding Buffer11 (BB11)10 ml10 ml
Clean Buffer 11 (CB11)55 ml55 ml
Wash Buffer 11 (WB11)12 ml12 ml
Elution Buffer (EB)25 ml25 ml
RNase A (10 mg/ml)1 ml0
Proteinase K (20 mg/ml)1 ml1 ml
Genomic Spin Columns with Collection Tubes50 each50 each


Citations

Liu Y, Chen Z, Jiang Z, et al. Biochemical characterization of a novel L-Asparaginase from Paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes[J]. International Journal of Biological Macromolecules,2017, 104(Pt A):1055-1063. 

Shi R , Liu Y , Mu Q , et al. Biochemical characterization of a novel L-asparaginase from Paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes[J]. International Journal of Biological Macromolecules, 2017, 96:93-99.

Zheng X , Liu H , Song L , et al. Phaeocystidibacter marisrubri sp. nov. a member of the family Cryomorphaceae isolated from Red Sea sediment[J]. International Journal of Systematic and Evolutionary Microbiology, 2015, 65(7):2199.

Duan D , Cheng T . Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high‐throughput sequencing[J]. Journal of Basic Microbiology, 2016, 57(4):302.

Cheng T Y , Liu G H . PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks[J]. Acta Parasitologica, 2017, 62(2):269-272.


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