pEASY Prokaryotic Expression Vectors
  • CE111-01.JPG
CE111-01.JPG

pEASY®-Blunt E1 Expression Kit


Product Details

pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.

• 5 minutes fast ligation of Pfu-amplified PCR products.
• Ampicillin resistance.
• T7 promoter primer and T7 terminator primer for sequencing.
• Bacteriophage T7lac promoter for high level expression.
• N-terminal 6×His tag for easy purification.
• Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
• E1 Expression Plasmid included as negative control.

Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70℃ for six months; Others at -20℃ for nine months.

Shipping
Dry ice (-70℃). 

Product Contents

ComponentCE111-01 (10 rxns)
pEASY®-Blunt E1 Expression Vector (15 ng/μl)10 μl
E1 Expression Plasmid (Negative Control) (15 ng/μl)10 μl
EControl Template (5 ng/μl)10 μl
EControl Forward Primer (10 μM)10 μl
EControl Reverse Primer (10 μM)10 μl
T7 Promoter Primer (10 μM)50 μl
T7 Terminator Primer (10 μM)50 μl
Trans1-T1 Phage Resistant Chemically Competent Cell5×100 μl

Citations

Yin Y R , Hu Q W , Xian W D , et al. Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501T[J]. 2017,110(3):429-436.

Yang N , Zhang D F , Tao Z , et al. Identification of a novel class B scavenger receptor homologue in Portunus trituberculatus: Molecular cloning and microbial ligand binding[J]. Fish & Shellfish Immunology, 2016, 58:73-81.

Liang M , Yang W , Su S , et al. Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrus[J]. Molecular Genetics and Genomics, 2017, 292(2):325-341.

Ju Z , Cao D , Gao C , et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.

Li L , Zheng M , Long H , et al. Molecular Cloning and Characterization of Two Genes Encoding Tryptophan Decarboxylase from Aegilops variabilis with Resistance to the Cereal Cyst Nematode (Heterodera avenae) and Root-Knot Nematode (Meloidogyne naasi)[J]. Plant Molecular Biology Reporter, 2016:1-10.


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