Expression Competent Cells
  • CD811-01.JPG
CD811-01.JPG

TransB(DE3) Chemically Comptent Cell


Product Details

TransB(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to kanamycin (KanR) and tetracycline (TetR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F- ompT hsdSB(rB-mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR)

Features
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• KanR and TetR.
• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

Storage
at -70℃ for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70℃)

Citations

Feng H1, Zhang D, Sun Y, et al.Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties fromStreptomyces griseorubensJSD-1[J]. Applied Biochemistry and Biotechnology, 2015, 176(2):547-562.

Zhou Y , Zhang H , Bao H , et al. The lytic activity of recombinant phage lysin LysKΔamidase against staphylococcal strains associated with bovine and human infections in the Jiangsu province of China[J]. Research in Veterinary Science, 2017, 111(Complete):113-119.

Li T , Wang Q , Chen F , et al. Biochemical Characteristics of New Delhi Metallo-β-Lactamase-1 Show Unexpected Difference to Other MBLs[J]. PLOS ONE, 2013, 12, 8(4):e61914.


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