ProteinFind^® Anti-CD9 Mouse Monoclonal Antibody

CD9 belongs to the tetraspanin family of cell surface glycoprotein. CD9 is expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, and the surface of neurons and glial cells in the peripheral nervous system^[1-3]. In myoblasts, CD9 acts synergistically with CD81 and PTGFRN to inhibit muscle tube fusion during muscle regeneration. In macrophages, CD9 acts synergistically with CD81, β-1 and β-2 integrins to prevents macrophages from fusing into multinucleated giant cells^[4]. CD9 also plays an important role in many cell physiological processes, including differentiation, adhesion and signal transduction. And it plays a key role in inhibiting the movement and metastasis of cancer cells^{[5, 6]}. This product is the mouse anti-human CD9 monoclonal antibody, which is used for the specific detection of human CD9 by IF and FC.

Applicable Experiments and Dilution

· IF: 1:100 dilution is recommended.

· FC: 1:100-1:800 dilution is recommended.

Storage

PBS (pH7.4), 0.05% ProClin 300, 50% Glycerol; at -20℃ for two years, avoid repeated freeze-thawing.

Shipping

Dry ice (-70℃)

[1] Reyes R, Cardeñes B, Machado-Pineda Y, et al. Tetraspanin CD9: A Key Regulator of Cell Adhesion in the Immune System [J]. Front Immunol. 2018, 9: 863.

[2] Jennings LK, Crossno JT Jr, Fox CF, et al. Platelet p24/CD9, a member of the tetraspanin family of proteins [J]. Ann N Y Acad Sci. 1994, 714(1): 175-84.

[3] Nakamura Y, Iwamoto R, Mekada E. Expression and distribution of CD9 in myelin of the central and peripheral nervous systems [J]. Am J Pathol. 1996, 149(2): 575-83. 

[4] Takeda Y, Tachibana I, Miyado K, et al. Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear  phagocytes [J]. Journal of Cell Biology. 2003, 161(5): 945-56.

[5] Ikeyama S, Koyama M, Yamaoko M, et al. Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA [J]. J Exp Med. 1993, 177(5): 1231-7. 

[6] Masellis-Smith A, Shaw AR. CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin [J]. J Immunol. 1994, 152(6): 2768-77.