Human IL-6 ELISA Kit

Interleukin-6 (IL-6) is a cytokine that has a multifunctional role in various biological processes, including immune  response, bone metabolism, hematopoiesis and tumor development. IL-6 has an α-helical structure with a molecular  weight of 22-28 kDa and can be easily phosphorylated and glycosylated. IL-6 is associated with inflammation and is a  typical cytokine that is secreted by both lymphoid cells (such as T cells and B cells) and certain non-lymphoid cells  (such as macrophages, monocytes, and mast cells) during infection or tissue damage. Its secretion plays an important  role in the host's defense system by clearing infected cells or damaged tissue. But overproduction of IL-6 can lead to  the development of chronic inflammatory diseases and cytokine storms. This kit uses the Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to measure the amount of IL-6 in human  serum, plasma, and cell culture supernatant. The ELISA microplate in the kit is precoated with a high-affinity  anti-human IL-6 antibody. The standard or test sample and a biotin-labeled anti-human IL-6 detection antibody are  added to the microplate wells at the same time. After incubation, IL-6 present in the sample will be bound specifically  to the precoated antibody and the detection antibody. After washing, a horseradish peroxidase-labeled streptavidin  (Streptavidin-HRP) is added to the microplate wells and incubated. Biotin and streptavidin on the detection antibody  produces the "coated antibody-human IL-6 protein-detection antibody-Streptavidin-HRP" immune complex through  high-strength non-covalent binding. After washing again, the chromogenic substrate TMB is added to the wells. HRP  catalyzes the TMB substrate to create blue coloration, with intensity positively correlated with the concentration of  IL-6 in the sample. The reaction is stopped by adding stop solution, and the absorbance is measured at 450 nm  (reference wavelength 570-630 nm). A standard curve is drawn to calculate the concentration of IL-6 in the sample  based on the absorbance value. This kit is highly specific, has high detection sensitivity and is convenient to use.

Suitable Sample Types 

Cell culture supernates, serum, plasma, etc.

Storage

at 2~8°C in the dark for one year.

Shipping
Ice bag(4°C)