Human IL-10 ELISA Kit

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a pleiotropic cytokine that can  exert immunosuppressive or immunostimulatory effects in various types of cells. IL-10 is a homodimeric secretion,  mainly produced by monocytes, T cells (mainly Th1 cells), B cells, NK cells, and macrophages. IL-10 has broad  anti-inflammatory activity and inhibits the expression of many pro-inflammatory factors, chemokines, and chemokine  receptors. IL-10 inhibits the production of IFN-γ and IL-2 by Th1 lymphocytes and the production of IL-4 and IL-5  by Th2 lymphocytes. IL-10 can also inhibit the expression of cell surface antigens, such as CD23, CD80, CD86, etc. This kit uses Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to measure the amount of IL-10 in human  serum, plasma, and cell culture supernatant. The ELISA microplate in the kit is precoated with a high-affinity  anti-human IL-10 antibody. The standard or test sample is added to the microplate wells. After incubation, IL-10 present in the sample will be bound specifically to the precoated antibody on the microplate wells. After washing,  biotin-labeled anti-human IL-10 detection antibody is added to the microplate wells. After re-incubation, the detection  antibody will be bound specifically to IL-10 anchored on the microplate wells. Subsequently, the horseradish  peroxidase-labeled streptavidin (Streptavidin-HRP) is added to the microplate wells and incubated. Biotin and  streptavidin on the detection antibody produces the "coated antibody-human IL-10 protein-detection  antibody-Streptavidin-HRP" immune complex through high-strength non-covalent binding. After washing again, the  chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to create blue coloration, with  intensity positively correlated with the concentration of IL-10 in the sample. The reaction is stopped by adding stop  solution, and the absorbance is measured at 450 nm (reference wavelength 570-630 nm). A standard curve is drawn to  calculate the concentration of IL-10 in the sample based on the absorbance value. This kit is highly specific, has high  detection sensitivity and is convenient to use.

Suitable Sample Types 

Cell culture supernates, serum, plasma, etc.

Storage

at 2~8°C in the dark for one year.

Shipping
Ice bag(4°C)