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DNA Polymerase
  • AP221.jpg
AP221.jpg

TransStart® FastPfu DNA Polymerase


Product Details

TransStart® FastPfu DNA Polymerase is a hot start high-fidelity DNA polymerase used for fast PCR. Offering a high

amplification efficiency and high extension rate (4 kb/min, it is 8 times that of ordinary Pfu enzymes), TransStart® FastPfu

DNA Polymerase amends the defects of low amplification efficiency, low yield and low extension rate (0.5 kb/min) in common

Pfu polymerase, and greatly shortens reaction time.

• Offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Blunt-end PCR products can be directly cloned into pEASY®-Blunt vectors.

• Amplification of genomic DNA fragment up to 15 kb.

• Amplification of plasmid DNA fragment up to 20 kb.


Features

• Hot start, high specificity.

• High amplification efficiency.

• Fast and high fidelity.


Applications

• Amplifies complex and high GC/ AT templates.

• High fidelity and fast PCR, blunt end cloning, site-directed mutagenesis.

• Amplifies long fragment.


Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)


Product Contents


ComponentAP221-01/11AP221-02/12AP221-03/13
TransStart® FastPfu DNA Polymerase250 U×1500 U×1500 U×6
TransStart® FastPfu Buffer1.2 ml×11.2 ml ×21.2 ml ×12
2.5 mM dNTPs- / 500 μl×1- / 1 ml ×1- / 1 ml ×6
6×DNA Loading Buffer500 μl×11 ml ×11 ml ×2
50 mM MgSO4200 μl×1400 μl ×11 ml ×1
Complimentary Component200 μl×1400 μl×11 ml ×1
PCR Stimulant


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