Direct PCR
  • AD201-01.JPG
AD201-01.JPG

TransDirect® Animal Tissue PCR Kit


Product Details

TransDirect® Animal Tissue PCR Kit uses a unique lysis buffer to lyse animal tissues (fresh or frozen) and blood. The resulting lysate without purification can be directly used as PCR template. 2×TransDirect® PCR SuperMix (+dye) is highly resistant to various PCR inhibitors present in animal tissues. PCR product can be directly used for gel electrophoresis.

Applications
• Direct amplification from unpurified lysate. Suitable for high throughput applications.
• Suitable for mammalian cells, saliva, hair shaft, animal tissues and blood.
• Amplification of genomic DNA fragment up to 3 kb.

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAD201-01AD201-02
AD1 Buffer4 ml20 ml
AD2 Buffer1 ml5 ml
AD3 Buffer4 ml2×10 ml
TransDirect® PCR SuperMix (+dye)1 ml5×1 ml
Nuclease-free Water5 ml25 ml


Citations

Chen Y , Hu W , Lu Y , et al. A TALEN-based specific transcript knock-down of PIWIL2 suppresses cell growth in HepG2 tumor cell[J]. Cell Proliferation, 2014, 47(5):448-456.

Zhao DD, Zhai YH, Liu Y, et al. Involvement of aurantiactinomyxon in the life cycle of Thelohanellus testudineus (Cnidaria: Myxosporea) from allogynogenetic gibel carp Carassius auratus gibelio, with morphological, ultrastructural, and molecular analysis[J].Parasitology Research, 2017,116(9):2449-2456.

Zhao D , Borkhanuddin M H, Wang W, et al. The life cycle of Thelohanellus kitauei (Myxozoa: Myxosporea) infecting common carp (Cyprinus carpio) involves aurantiactinomyxon in Branchiura sowerbyi[J]. Parasitology Research, 2016, 115(11):4317-4325.

Qin S, Zhang Q , Zhang L . Effect of OPG gene mutation on protein expression and biological activity in osteoporosis[J]. Experimental & Therapeutic Medicine, 2017, 14(2):1475-1480.

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