Universal Nuclease, a genetically engineered endonuclease from Serratia marcescens, is a kind of no-specific endonuclease. The enzyme is produced and purified from Escherichia coli. It can digest nucleic acids to 2 to 5 bases 5’ monophosphate terminated oligonucleotides without selectivity. Therefore, this product can efficiently degrade all kinds of DNA and RNA (double stranded, single stranded, linear and circular) but without proteolytic activity, which is widely used to remove nucleic acid residues and contamination in biological products.

Applications 

 · For protein purification or protein extraction from tissue cell samples, this product is used to remove nucleic acid contamination and reduce sample viscosity.

 · Add Universal Nuclease to cell or bacterial lysate to remove nucleic acids from crude extracts, reduce solution viscosity, and increase protein yield; 

 · Remove the effect of negatively charged nucleic acids on two-dimensional SDS-PAGE protein samples, improve protein separation and enhance two-dimensional electrophoresis resolution;

 · This product is used in vaccine production, virus purification, protein and polysaccharide pharmaceutical industry as a host residual nucleic acid removal reagent, reducing host nucleic acid residue to pg level and improving the efficacy and safety of biological products; 

 · Reduce clumping of human peripheral blood single cells (PBMCs) stored in cell therapy and vaccine studies

Storage

at -20℃ for two years

Shipping

Dry ice (-70℃)