pEASY Cloning Vectors
  • CT301-01.JPG
CT301-01.JPG

pEASY® -T3 Cloning Kit


Product Details

pEASY®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.

•5 minutes fast ligation of Taq-amplified PCR products.
•Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter and SP6 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage
Competent Cell at -70°C for six months; others at -20°C for nine months

Shipping
Dry ice (-70℃)

Product Contents


ComponentCT301-01 (20 rxns)CT301-02 (60 rxns)
pEASY ®--T3 Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl


Citations

Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching[J]. Nature Biotechnology, 2013, 31(9):848-852.

Wang J, Wang L, Feng G, et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos[J]. Cell, 2018, 13, 175(7):1887-1901.

Zhang YC, Liao JY, Li ZY, et al. Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice[J]. Genome Biology, 2014, 15(12):512.

Chen Z H , Wang W T , Huang W , et al. The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway[J]. Cell Death and Differentiation,  2017, 24(2):212-224.

Peng S, Feng Y, Wang Y, et al. Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years[J]. J Hazard Mater. 2017, 15(340):16-25. 


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