pEASY®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.
•5 minutes fast ligation of Taq-amplified PCR products.
•Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter and SP6 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for nine months
Shipping
Dry ice (-70℃)
Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching[J]. Nature Biotechnology, 2013, 31(9):848-852.
Wang J, Wang L, Feng G, et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos[J]. Cell, 2018, 13, 175(7):1887-1901.
Zhang YC, Liao JY, Li ZY, et al. Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice[J]. Genome Biology, 2014, 15(12):512.
Chen Z H , Wang W T , Huang W , et al. The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway[J]. Cell Death and Differentiation, 2017, 24(2):212-224.
Peng S, Feng Y, Wang Y, et al. Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years[J]. J Hazard Mater. 2017, 15(340):16-25.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
