Trans109 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA).
Genotype
endA1 recA1 gyrA96 thi-1 hsdR17 (rk-, mk+) relA1 supE44 D (lac-proAB) [F′traD36 proAB laqIqZΔM15]
Features
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• The lowest homologous recombination favorable for plasmid DNA preparation.
• Routine cloning.
• Blue/white selection.
Storage
at -70°C for six months. Do not store in liquid nitrogen
Shipping
Dry ice (-70℃)
Li BJ, Wang H, Gong T, et al. Improving 10-deacetylbaccatin III-10-b-Oacetyltransferase catalytic fitness for Taxol production[J]. Nature Communications,2017, 18(8):15544.
Guo Z , Kang S , Zhu X , et al. Down-regulation of a novel ABC transporter gene (Pxwhite) is associated with Cry1Ac resistance in the diamondback moth, Plutella xylostella (L.)[J]. Insect Biochemistry and Molecular Biology, 2015, 59:30-40.
Zhang M , Jiang, Shaoâtong, Zheng Z , et al. Cloning, expression, and characterization of a novel xylose reductase from Rhizopus oryzae[J]. Journal of Basic Microbiology, 2015, 55(7):907-21.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
