Cell Detection
  • FR201-01.JPG
FR201-01.JPG

TransDetect® Double-Luciferase Reporter Assay Kit


Product Details

TransDetect® Double-Luciferase Reporter Assay Kit provides an efficient method to perform dual-reporter assays. In the assay, the activities of firefly (Photinus pyralis) and Renilla (sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured firstly by adding Luciferase Reaction Reagent to generate a luminescent signal. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated by adding Luciferase Reaction Reagent II to the same tube.

Storage
at -20℃ in dark for one year. Prepared luciferase assay reagents (Luciferase Reaction Reagent and Luciferase Reaction Reagent II) should be stored in aliquots in dark at -20℃ for one month or at -70℃ for one year.

Shipping
Dry ice (-70℃)

Product Contents

ComponentFR201-01 (50 rxns)FR201-02 (200 rxns)
Luciferase Reaction Buffer5 ml20 ml
Luciferase Reaction Substrate (Lyophilized)1 vial4 vials
Luciferase Reaction Buffer II5 ml20 ml
Luciferase Reaction Substrate II (50×)100 μl400 μl
Cell Lysis Buffer (5×)5 ml20 ml

Citations

Zhou W , Yao J , Wang G , et al. PKCζ phosphorylates TRAF2 to protect against intestinal ischemia–reperfusion–induced injury[J]. Cell Death & Disease, 2017, 8(7):e2935.

Shan W , Gao L , Zeng W , et al. Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease[J]. Cell Death and Disease, 2015, 6(7):e1833.

Xiong Y , Shi L , Wang L , et al. Activation of sirtuin 1 by catalpol-induced down-regulation of microRNA-132 attenuates endoplasmic reticulum stress in colitis[J]. Pharmacological Research, 2017:S1043661817303237.

Wang M , Gao Y , Qu P , et al. Sperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine[J]. Scientific Reports, 2017, 7(1):13403.

Yang Z , Wang X , Yang J , et al. Aberrant CpG Methylation Mediates Abnormal Transcription ofMAO-AInduced by Acute and Chronicl-3,4-Dihydroxyphenylalanine Administration in SH-SY5Y Neuronal Cells[J]. Neurotoxicity Research, 2017, 31(3):334-347.


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