Cell Detection
  • FR201-01.JPG

TransDetect® Double-Luciferase Reporter Assay Kit

Product Details

TransDetect® Double-Luciferase Reporter Assay Kit provides an efficient method to perform dual-reporter assays. In the assay, the activities of firefly (Photinus pyralis) and Renilla (sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured firstly by adding Luciferase Reaction Reagent to generate a luminescent signal. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated by adding Luciferase Reaction Reagent II to the same tube.

at -20℃ in dark for one year. Prepared luciferase assay reagents (Luciferase Reaction Reagent and Luciferase Reaction Reagent II) should be stored in aliquots in dark at -20℃ for one month or at -70℃ for one year.

Dry ice (-70℃)

Product Contents

ComponentFR201-01 (50 rxns)FR201-02 (200 rxns)
Luciferase Reaction Buffer5 ml20 ml
Luciferase Reaction Substrate (Lyophilized)1 vial4 vials
Luciferase Reaction Buffer II5 ml20 ml
Luciferase Reaction Substrate II (50×)100 μl400 μl
Cell Lysis Buffer (5×)5 ml20 ml


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