Firefly luciferase and Renilla luciferase can catalyze the oxidation of luciferin or coelenterazine to form oxyluciferin or coelenteramide, respectively, and produce bioluminescence in the process. TransDetect® Double-Luciferase Reporter Assay Kit first uses luciferin as a substrate to detect the activity of the firefly luciferase reporter gene. Then, while quenching the fluorescence reaction, the activity of the Renilla luciferase reporter gene was detected by using coelenterazine as a substrate. It has the characteristics of rapid detection, high sensitivity, wide detection range, and no interference with the endogenous activity of cells.
Storage
The kit stored at -20°C for one year. Cell Lysis Buffer can be stored at -20°C for one year. Prepared Luciferase Reaction Reagent should be stored in aliquots in dark at -20°C for one month or at -70°C for one year.
Shipping
Dry ice (-70℃)
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Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
