The TransDetect® Cell LIVE/DEAD Viability/Cytotoxicity Detection Kit provides a simple, high-specificity and low-toxicity way to
distinguish between live and dead cells by combining Calcein-AM and Propidium iodide (PI). Quantification or sorting can be
performed by flow cytometry.
Calcein-AM is a low-cytotoxicity non-fluorescent dye, which easily permeates intact live cells. In live cells, the Calcein-AM is
converted to green fluorescent calcein with excitation and emission wavelengths of 490/515 nm by intracellular esterase digestion.
PI is excluded by intact plasma membrane of live cells. It is a DNA-binding fluorescent dye with excitation and emission
wavelengths of 535/617 nm， producing a bright red fluorescence in dead cells with broken membrane.
• Higher specificity.
• More simplicity.
• Lower cytotoxicity.
Storage
at -20℃ in the dark for up to one year.
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
