The Annexin V-EGFP/PI Cell Apoptosis Kit provides a rapid and sensitive method for early apoptosis detection. In normal cells, the
membrane phospholipid phosphatidylserine (PS) is located on the cytoplasmic surface of the membrane. In apoptotic cells, PS is
translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment.
The EGFP-conjugated Annexin V, a Ca2+ dependent phospholipid-binding protein, can bind specifically to the exposed PS. Propidium
iodide (PI) is a nucleic acid binding dye, which binds tightly to the nucleic acids in the cells and stains the cells with red fluorescence.
PI is impermeant to live cells and early apoptotic cells, so the combination of Annexin V-EGFP and PI staining allows the
differentiation among different stage of apoptotic cells and necrosis cells. Compared with FITC, EGFP is brighter and more
photo-stable. Because Annexin V-EGFP is a fusion protein with a 1:1 binding ratio of EGFP to PS, this kit can also be used for
quantitative detection.
Storage
at 2-8℃ in dark for one year.
Shipping
Ice bag (4℃)
Li S , Jiang Z , Xu W , et al. FIP-sch2, a new fungal immunomodulatory protein fromStachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells[J]. Applied Microbiology and Biotechnology, 2017, 101(8):3227-3235.
Liu G , Chen M , Yu C , et al. Molecular cloning, characterization and functional analysis of a putative mitogen-activated protein kinase kinase kinase 4 (MEKK4) from blood clam, Tegillarca granosa[J]. Fish & Shellfish Immunology, 2017, 66:372-381.
Li S , Jiang Z , Sun L , et al. Characterization of a new fungal immunomodulatory protein, FIP-dsq2 from, Dichomitus squalens[J]. Journal of Biotechnology, 2017, 246(Complete):45-51.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
