The kit is designed for detecting the ability of cell proliferation with the thymidine analog 5-ethynyl-2'-deoxyuridine (EdU). In the process of DNA replication, EdU can be inserted into the newly synthesized DNA double-stranded structure, and then EdU can be labeled by fluorescent groups by click reaction. Flow cytometry method is used to detect the DNA replication activity of the S phase according to the fluorescence intensity, so as to detect the cell proliferation ability. The maximum excitation wavelength and emission wavelength of 488 Fluorophore are 494 nm and 520 nm respectively.
Compared with traditional BrdU assay methods, this kit does not require additional steps such as antibody labeling, is easy to operate, has high sensitivity and specificity. It is suitable for drug screening, cell proliferation assay, cytotoxicity assay and other experiments.
Storage
at 2-8℃ in the dark for one year
Shipping
ice bag (4℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
