Peripheral blood mononuclear cells (mainly lymphocytes) differ in size, morphology, and density from other cells. The density of red blood cells and granulocytes is relatively high, about 1.090 g/ml, the density of platelets is 1.030 ~ 1.035 g/ml, while the density of mononuclear cells is 1.075 ~ 1.090 g/ml. This product is a sterile, nearly isotonic, density of (1.077±0.001) g/ml (20℃) glucan and meglumin diatrizoate solution. When density gradient centrifugation of human anticoagulant blood is performed with this product, red blood cells and granulocytes with high density will sink in this separation solution, while the density of peripheral blood mononuclear cells (mainly lymphocytes) is slightly lower than the separation solution and will float in this separation solution, so lymphocytes with higher purity could be obtained. This product is ready-to-use. The isolated lymphocytes can be used for in vitro culture and immunological testing.
Storage
at room temperature (15-25℃) away from light for two years.
Shipping
at room temperature.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
