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  • GD201-01.JPG
GD201-01.JPG

DNase I (RNase-free )


Product Details

Deoxyribonuclease I (DNase I) is an endonuclease that degrades doubleand single-strand DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ cuts randomly double strand DNA at any site, DNase I with Mn2+ cuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or form blunt-end.

Storage
at -20˚C for one year.

Shipping
Dry ice (-70℃).

Product Contents

ComponentGD201-01
DNase I (3 units/μl)1500 units
10×DNase I Reaction Buffer2×1 ml
200 mM EDTA1 ml

References

1.Zhang G, Wang Z, Du Z, et al. mTOR Regulates Phase Separation of PGL Granules to ModulateTheir Autophagic Degradation[J] Cell, 2018, 174(6):1492-1506.
2.Liu Z Y , Yao X Q , Zhang Q , et al. Modulation of the Acetone/Butanol Ratio during Fermentation of Corn StoverDerived Hydrolysate by Clostridium beijerinckii Strain NCIMB 8052[J]. Applied & Environmental Microbiology, 2017, 83(7):AEM.03386-16.
3.Wang S , Wang C , Zhang X X , et al. Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in cabbage[J]. Plant Physiology & Biochemistry Ppb, 2016, 105:224-232.

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