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qPCR and qRT-PCR

  • TransScript<sup>®</sup>  II  Green One-Step qRT-PCR SuperMix
TransScript<sup>®</sup>  II  Green One-Step qRT-PCR SuperMix

TransScript® II Green One-Step qRT-PCR SuperMix


  • Product Introduction
  • Manual Download
  • References


Description

TransScript® II Green One-Step qRT-PCR SuperMix combines the first-strand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. TransScript® II Green One-Step qRT-PCR SuperMix contains all the necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene specific primers.


Highlights

• 5 minutes cDNA synthesis
• cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
• Passive reference dyes are provided for different qPCR instruments.


Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• Viral RNA and trace RNA detection


Passive Reference

Dye Selection

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q


Storage

at -20℃ for one year


Kit Contents


Component

AQ311-01

AQ311-02

TransScript® II One-Step RT/RI Enzyme Mix

40 μl

160 μl

TransStart® Tip Green qPCR SuperMix (2×)

1 ml

4×1 ml

Passive Reference Dye (50×)

40 μl

160 μl

RNase-free Water

1 ml

4×1 ml


Literature Journal IF Author Date Link
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