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qPCR and qRT-PCR

  • TransStart<sup>®</sup> Tip Green qPCR SuperMix
TransStart<sup>®</sup> Tip Green qPCR SuperMix

TransStart® Tip Green qPCR SuperMix


  • Product Introduction
  • Manual Download
  • References

Description

TransStart® Tip Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a novel TransStart® TipTaq DNA Polymerase , unique hot start reagents (DNA binding proteins combined with unique chemical), optimized double cation buffer, SYBR Green I, dNTPs, PCR Enhancerm and PCR stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.


Highlights

• A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart®TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.


Passive Reference

Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q


Storage

at -20℃ for one year


Kit Contents


Component

AQ141-01

AQ141-02

AQ141-03

AQ141-04

TransStart® Tip Green qPCR SuperMix

1 ml

5×1 ml

15×1 ml

25×1 ml

Passive Reference Dye (50×)

40 μl

200 μl

600 μl

1 ml

ddH2O

1 ml

5 ml

3×5 ml

5×5 ml




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