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RT-PCR

  • TransScript<sup>®</sup>  Fly First-Strand cDNA Synthesis SuperMix
TransScript<sup>®</sup>  Fly First-Strand cDNA Synthesis SuperMix

TransScript® Fly First-Strand cDNA Synthesis SuperMix


  • Product Introduction
  • Manual Download
  • References

Description

TransScript® Fly RT is generated by site mutations. It provides high affinity to RNA template with fast extension rate. The cDNA is efficiently synthesized by TransScript® Fly RT/RI Enzyme Mix and 2×TS Fly Reaction Mix. The entire reverse transcription can be completed within 5 minutes.


Highlights

• 5 minutes reverse transcription.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.

• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.

• cDNA up to 12 kb.


Applications

Multiple copy and low copy gene fast detection


Storage

at -20℃ for two year


Kit Contents


Component

AF301-02

AF301-03

TransScript® Fly RT/RI Enzyme Mix

50 µl

100 µl

2×TS Fly Reaction Mix

500 µl

1 ml

Random Primer(N9) (0.1 µg/µl)

50 µl

100 µl

Anchored Oligo(dT)18 Primer (0.5 µg/µl)

50 µl

100 µl

RNase-free Water

500 µl

1 ml


Literature Journal IF Author Date Link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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