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RT-PCR

  • TransScript<sup>®</sup> Reverse Transcriptase
  • TransScript<sup>®</sup> Reverse Transcriptase
TransScript<sup>®</sup> Reverse TranscriptaseTransScript<sup>®</sup> Reverse Transcriptase

TransScript® Reverse Transcriptase


  • Product Introduction
  • Manual Download
  • References


Description

TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.

• cDNA up to 12 kb.

Applications

• First-strand cDNA synthesis
• Multiple copy and low copy gene detection

Unit Definition

One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

5×TS RT Buffer

250 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4

Storage

at -20℃ for two year

Kit Contents

Component

AT101-02

AT101-03

TransScript® RT

10000 U

5×10000 U

5×TS RT Buffer

200 μl

5×200 μl

Anchored Oligo(dT)18 Primer (0.5 µg/µl)

50 μl

250 μl

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