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PCR Enzyme

  • TransTaq<sup>®</sup> DNA Polymerase High Fidelity(HiFi)
  • TransTaq<sup>®</sup> DNA Polymerase High Fidelity(HiFi)
TransTaq<sup>®</sup> DNA Polymerase High Fidelity(HiFi)TransTaq<sup>®</sup> DNA Polymerase High Fidelity(HiFi)

TransTaq® DNA Polymerase High Fidelity(HiFi)


  • Product Introduction
  • Manual Download
  • References

Description

TransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq®HiFi DNA Polymerase provides higher specificity and higher amplification efficiency thanTransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA andTransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

Highlights

• TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared toEasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned intopEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.

Applications

• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR

Unit Definition

One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

Quality Control

TransTaq® HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch ofTransTaq® HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.

Storage

at -20 ℃ for two years

Kit Contents

Component

AP131-01/11

AP131-02/12

AP131-03/13

TransTaq® HiFi DNA Polymerase

250 U×1

500 U×1

500 U×6

10×TransTaq® HiFi Buffer I

1.2 ml×1

1.2 ml×1

1.2 ml×6

10×TransTaq® HiFi Buffer II

1.2 ml×1

1.2 ml×1

1.2 ml×6

2.5 mM dNTP

-/400 μl×1

-/800 μl×1

-/800 μl×6

10×GC Enhancer

200 μl×1

400 μl×1

1 ml×1

6×DNA Loading Buffer

500 μl×1

1 ml×1

1 ml×2

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