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PCR Enzyme

  • TransTaq<sup>®</sup>-T DNA Polymerase
  • TransTaq<sup>®</sup>-T DNA Polymerase
  • TransTaq<sup>®</sup>-T DNA Polymerase
  • TransTaq<sup>®</sup>-T DNA Polymerase
  • TransTaq<sup>®</sup>-T DNA Polymerase
  • TransTaq<sup>®</sup>-T DNA Polymerase
TransTaq<sup>®</sup>-T DNA PolymeraseTransTaq<sup>®</sup>-T DNA PolymeraseTransTaq<sup>®</sup>-T DNA PolymeraseTransTaq<sup>®</sup>-T DNA PolymeraseTransTaq<sup>®</sup>-T DNA PolymeraseTransTaq<sup>®</sup>-T DNA Polymerase

TransTaq®-T DNA Polymerase


  • Product Introduction
  • Manual Download
  • References

Description

TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that fromEasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.

Highlights

• TransTaq®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent“A”can be generated at the 3’end of the PCR product. PCR products can be directly cloned intopEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.

Applications

• Complex templates
• TA cloning

Unit Definition

One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

Quality Control

TransTaq®-T DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq®-T DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

Storage

at -20 ℃ for two years


Kit Contents

Component

AP122-01/11

AP122-02/12

AP122-03/13

TransTaq®-T DNA Polymerase

250 U×1

500 U×1

500 U×6

10×TransTaq®-T Buffer

1.2 ml ×1

1.2 ml ×1

1.2 ml ×6

2.5 mM dNTPs

- / 400 µl ×1

- / 800 µl ×1

- / 800 µl ×6

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2

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