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  • DNase I (RNase-free )
DNase I (RNase-free )

DNase I (RNase-free )


  • Product Introduction
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Description

Deoxyribonuclease I (DNase I) is an endonuclease that degrades doubleand single-strand DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ cuts randomly double strand DNA at any site, DNase I with Mn2+ cuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or form blunt-end.


Unit Definition

One unit is the amount of enzyme required to completely degrade 1 μg pBR322 plasmid DNA in 10 minutes at 37°C.


Source

Purified from bovine pancreas


Storage

at -20°C for one year


Literature Journal IF Author Date Link
Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-derived Hydrolysate by Clostridium beijerinckii NCIMB 8052 applied and environmental microbiology 3.8229 Zi-Yong Liu,et al. 2017Jan The original link
Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in cabbage plant physiology and biochemistry 2.7239 Shuai Wang,et al. 2016Aug The original link
Effects of starvation and feeding on blood chemistry, fatty acid composition and expression of vitellogenin and fatty acid?binding protein genes in female swimming crab Portunus trituberculatus broodstock Fisheries Science 0.83899 Liyun Ding,et al 2017 May The original link
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