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Cell Detection

  • TransDetect<sup>®</sup> Annexin V-EGFP/PI Cell  Apoptosis Detection Kit
TransDetect<sup>®</sup> Annexin V-EGFP/PI Cell  Apoptosis Detection Kit

TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit


  • Product Introduction
  • Manual Download
  • References

Description

The Annexin V-EGFP/PI Cell Apoptosis Kit provides a rapid and sensitive method for early apoptosis detection. In normal cells, the membrane phospholipid phosphatidylserine (PS) is located on the cytoplasmic surface of the membrane. In apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. The EGFP-conjugated Annexin V, a Ca2+ dependent phospholipid-binding protein, can bind specifically to the exposed PS. Propidium iodide (PI) is a nucleic acid binding dye, which binds tightly to the nucleic acids in the cells and stains the cells with red fluorescence. PI is impermeant to live cells and early apoptotic cells, so the combination of Annexin V-EGFP and PI staining allows the differentiation among different stage of apoptotic cells and necrosis cells. Compared with FITC, EGFP is brighter and more photo-stable. Because Annexin V-EGFP is a fusion protein with a 1:1 binding ratio of EGFP to PS, this kit can also be used for quantitative detection.


Storage

at 4°C in dark for one year


Kit Content


Component

FA111-01 (25 rxns)

FA111-02 (50 rxns)

Annexin V-EGFP

125 μl

250 μl

Propidium Iodide (PI)

125 μl

250 μl

1× Annexin V Binding Buffer

12.5 ml

2×12.5 ml



Literature Journal IF Author Date Link
FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells applied microbiology and biotechnology 3.376 Li S, et al. Institute of Food Science and Technol 2017 Jan The original link
Molecular cloning, characterization and functional analysis of a putative mitogen-activated protein kinase kinase kinase 4 (MEKK4) from blood clam Tegillarca granosa Fish & Shellfish Immunology 3.148 Guosheng Liu, et al. 2017 July The original link
Characterization of a New Fungal Immunomodulatory Protein, FIP-dsq2 from Dichomitus squalens. journal of biotechnology 2.667 Li S, et al. Institute of Food Science and Technol 2017 Feb The original link
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