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Cell Detection

  • TransDetect<sup>®</sup> Cell Counting Kit (CCK)
TransDetect<sup>®</sup> Cell Counting Kit (CCK)

TransDetect® Cell Counting Kit (CCK)


  • Product Introduction
  • Manual Download
  • References

Description

TransDetect® Cell Counting Kit (CCK) is designed for cell proliferation assays as well as cytotoxicity assays by utilizing a water-soluble tetrazolium salt. The salt can be reduced to an orange water-soluble formazan by mitochondrial dehydrogenase in the presence of an electron coupling reagent 1-Methoxy PMS. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. Faster cell proliferation, lower cytotoxicity, and more cell number produce deeper color. The depth of the dye is directly proportional to the number of living cells. The toxicity of CCK solution is so low that the same cells can be used for other assays after the CCK assay is completed. Compared with MTT, XTT, MST and WST-1, this method provides higher sensitivity and broader linear range. It is suitable for drug screening, cell proliferation test, cytotoxicity assay and drug sensitivity test.

Storage

at 2-8°C in dark for one year or at -20°C in dark for two years

Shipping Condition

Dry ice (-70°C)

Kit Content

Component

FC101-01

FC101-02 FC101-03

FC101-04

CCK Solution

1 ml

5 ml

2×5 ml

6×5 ml



Literature Journal IF Author Date Link
Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion(doi: 10.1093/nar/gkx178) nucleic acids research 9.202 Li P, et al. Institute of Virology and AIDS Resear 2017 Mar The original link
GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer cancer letters 5.992 Gu J, et al. Department of General Surgery, Ruijin 2016 Oct The original link
A novel approach to prepare a tissue engineering decellularized valve scaffold with poly(ethylene glycol)–poly(ε-caprolactone) RSC Advances 3.84 Zhigang Zhu, et al. 2016 Jan The original link
LncRNA UCA1 promotes renal cell carcinoma proliferation through epigenetically repressing p21 expression and negatively regulating miR-495 Tumor biology 3.65 Yun Lu et al Department of Nephrology, The Affilia 2017 May The original link
FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells applied microbiology and biotechnology 3.42 Shuying Li,et al. 2017Apr The original link

CONTACT US

Contacts:Shuyi Yan

Skype:TransGen Biotech Co., LTD

Telephone:0086-10-57815027

E-mail:info@transgenbiotech.com

Address: Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China


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