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Protein Extraction

  • ProteinExt<sup>®</sup> Mammalian Membrane Protein  Extraction Kit
ProteinExt<sup>®</sup> Mammalian Membrane Protein  Extraction Kit

ProteinExt® Mammalian Membrane Protein Extraction Kit


  • Product Introduction
  • Manual Download
  • References

Description

ProteinExtTM Mammalian Membrane Extraction Kit provides a simple and efficient method to extract membrane proteins from mammalian cells and tissues. Native proteins can be obtained within 70 minutes without ultracentrifugation. Up to 90% efficiency for membrane proteins have at least 1-2 transmembrane domains. The extracted protein is suitable for a variety of downstream applications, including SDS-PAGE, Western Blot, ELISA, and enzyme-activity assays.


Storage

ProteinSafe™ Protease Inhibitor Cocktail at -20°C for one year, others at 2-8°C for one year


Kit Content


Component

DE301-01 (50 rxns)

Membrane Protein Extraction Buffer I (MPEB I)

50 ml

Membrane Protein Extraction Buffer II (MPEB II)

7.5 ml

Membrane Protein Extraction Buffer III (MPEB III)

15 ml

ProteinSafeTM Protease Inhibitor Cocktail, EDTA-free (100×)

1 ml



Literature Journal IF Author Date Link
Progesterone Receptor Membrane Component 1 Mediates Progesterone-Induced Suppression of Oocyte Meiotic Prophase I and Primordial Folliculogenesis Scientific Reports 5.228 Guo M, et al. Department of Laboratory Animal Scie 2016 Nov The original link
Complex Roles of Annexin A2 in Host Blood?CBrain Barrier Invasion by Cryptococcus neoformans CNS NEUROSCIENCE & THERAPEUTICS 4.019 Fang W, et al. PLA Key Laboratory of Mycosis, Depa 2017 Jan The original link
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