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Next Generation Sequencing

  • TransNGS<sup>TM</sup> rRNA Depletion Kit (Human/Mouse/Rat)
TransNGS<sup>TM</sup> rRNA Depletion Kit (Human/Mouse/Rat)

TransNGSTM rRNA Depletion Kit (Human/Mouse/Rat)


  • Product Introduction
  • Manual Download
  • References

Description

TransNGS™ rRNA Depletion Kit (Human/Mouse/Rat) depletes ribosomal RNA from human/mouse/rat total RNA by RNase H digestion, while retains messenger RNA and non-coding RNA. The depleted ribosomal RNA contains both cytoplasmic ribosomal RNA (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis.

Features:

• Remove up to 99% ribosomal RNA from human/mouse/rat total RNA.

• Control qPCR Primer Sets are provided to monitor the depletion efficiency of ribosomal RNA and the retention rate of

non-ribosomal RNA.

Storage

at-20°C for one year

Kit Content

Component

KD101-01 (6 rxns)

KD101-02 (24 rxns)

KD101-03 (96 rxns)

rRNA Probe (H/M/R)

12 μl

48 μl

192 μl

E.coli RNase H

6 μl

24 μl

96 μl

DNase I

30 μl

120 μl

480 μl

5×Hybridization Buffer

18 μl

72 μl

288 μl

10×RNase H Reaction Buffer

12 μl

48 μl

192 μl

10×DNase I Reaction Buffer

30 μl

120 μl

480 μl

RNase-free Water

500 μl

2×1 ml

8×1 ml

Control qPCR

Ribosomal RNA Primer Mix

12 μl

48 μl

192 μl

Non-ribosomal RNA Primer Mix

12 μl

48 μl

192 μl



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