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Plasmid DNA Purification and E. Coli Medium

  • EasyPure<sup>®</sup> Plasmid MiniPrep Kit
EasyPure<sup>®</sup> Plasmid MiniPrep Kit

EasyPure® Plasmid MiniPrep Kit

  • Product Introduction
  • Manual Download
  • References


EasyPure® Plasmid MiniPrep Kit uses a modified alkaline lysis method to isolate high-quality plasmid DNA from ≤20 ml (LB) or ≤4 ml (ArtMedia® Plasmid Culture) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit error-free visual identification of complete bacterial cell lysis and neutralization.

• Simple and fast: the whole procedure can be performed in 20 minutes.

• High yield: DNA yield up to 40 μg.

• Error-free visualization: colored buffers to visualize lysis and neutralization.


Restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection


RNase A at -20°C for one year; others at room temperature (15-25°C) for one year

Kit Content


EM101-01 (50 rxns)

EM101-02 (200 rxns)

Resuspension Buffer (RB)

15 ml

60 ml

Lysis Buffer (LB)

15 ml

60 ml

Neutralization Buffer (NB)

20 ml

80 ml

Wash Buffer (WB)

10 ml

2×20 ml

Elution Buffer (EB)

5 ml

10 ml

RNase A (10 mg/ml)

150 μl

600 μl

Mini-Plasmid Spin Column with Collection Tubes

50 each

2×100 each

Literature Journal IF Author Date Link
Identification of Tisp40 as an Essential Regulator of Renal Tubulointerstitial Fibrosis via TGF-β/Smads Pathway cellular physiology and biochemistry 5.104 Cheng-cheng Xiao,et al. 2017Jul The original link
Molecular characterization of microbial populations in full-scale biofilters treating iron, manganese and ammonia containing groundwater in Harbin, China Bioresource Technology 4.75 Li X, et al. State Key Laboratory of Urban Water 2013 Nov The original link
A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs Bioresource Technology 4.75 Jianmei Luo, et al Key Laboratory of Industrial Fe 2013 Jul The original link
Influence of the amoxicillin concentration on organics removal and microbial community structure in an anaerobic EGSB reactor treating with antibiotic wastewater Chemical Engineering Journal 4.321 Ling-Wei Meng, et al. 2015 Mar The original link
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi. PLoS One 4.092 Wang B. et al. Key Laboratory of Zoological Syste 2013 The original link