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Cloning Vectors

  • pEASY<sup>®</sup>-T1 Simple Cloning Kit
pEASY<sup>®</sup>-T1 Simple Cloning Kit

pEASY®-T1 Simple Cloning Kit


  • Product Introduction
  • Manual Download
  • References

Description

pEASY ®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products.

•5 minutes fast ligation of Taq-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•SR primer and M13 forward primer for sequencing.

•T7 promoter for in vitro transcription.

Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.


Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months


Kit Content


Component

CT111-01

CT111-02

(20 rxns)

(60 rxns)

pEASY ®-T1 Simple Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13Forward Primer (10 μM)

50 µl

150 µl

M13Reverse Primer (10 μM)

50 µl

150 µl

SR Primer (10 μM)

50 µl

150 µl

Trans1-T1 Phage Resistant Chemically Competent Cell

10×100 μl

30×100 μl




Literature Journal IF Author Date Link
A transgenic strategy for controlling plant bugs(Adelphocoris suturalis) through expression of double-stranded RNA homologous to fatty acyl-coenzyme A reductase in cotton new phytologist 7.21 Jing Luo,et al. 2017Jun The original link
A transposon-directed epigenetic change in ZmCCT underlies quantitative resistance to Gibberella stalk rot in maize new phytologist 7.2 Chao Wang et al National Maize Improvement Centre 2017 Jul The original link
Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification analytical chemistry 6.32 Yi Hu,et al. 2016Nov The original link
Nitrogen deposition mediates the effects and importance of chance in changing biodiversity Molecular Ecology 6.275 Ximei Zhang, et al. State Key Laboratory of Vegeta 2011 Jan The original link
Characterization of Cyclooxygenase-2 and its induction pathways in response to high lipid diet-induced inflammation in Larmichthys crocea Scientific Reports 5.578 Tianjiao Wang, et al. 2016 Feb The original link
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