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Fast Restriction Enzyme

  • FlyCut<sup>®</sup> SacII
  • FlyCut<sup>®</sup> SacII
FlyCut<sup>®</sup> SacIIFlyCut<sup>®</sup> SacII

FlyCut® SacII


  • Product Introduction
  • Manual Download
  • References

Description

FlyCut ®SacII is expressed and purified from E.coli that carries the recombinant SacII gene. The molecular weight is 32.1 kDa, with the recognition site at CCGC^GG The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.

 

Concentration

20,000 units/ml

 

Recognition Site

5’...CCGC↓GG...3’

3’...GG↑CGCC...5’

 

Applications

Genomic DNA, plasmid DNA, PCR product

 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

 

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

 

10×FlyCut® Buffer

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Storage

at -20℃ for two years

 

Kit Content

 

 

Component

JS201-01

JS201-02

FlyCut® SacII

1,000 units

2×1,000 units

10×FlyCut® Buffer

250 μl

500 μl

10×DNA Loading Buffer

1 ml

1 ml



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