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Description |
FlyCut ®SacII is expressed and purified from E.coli that carries the recombinant SacII gene. The molecular weight is 32.1 kDa, with the recognition site at CCGC^GG The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
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Concentration |
20,000 units/ml
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Recognition Site |
5’...CCGC↓GG...3’ 3’...GG↑CGCC...5’
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Applications |
Genomic DNA, plasmid DNA, PCR product
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Unit Definition |
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
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Storage Buffer |
20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol
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10×FlyCut® Buffer |
500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA
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Storage |
at -20℃ for two years
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Kit Content |
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Component |
JS201-01 |
JS201-02 |
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FlyCut® SacII |
1,000 units |
2×1,000 units |
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10×FlyCut® Buffer |
250 μl |
500 μl |
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10×DNA Loading Buffer |
1 ml |
1 ml |
| Literature | Journal | IF | Author | Date | Link |
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Contacts：Shuyi Yan
Skype：TransGen Biotech Co., LTD
Telephone：0086-10-57815027
E-mail：info@transgenbiotech.com
Address： Building No.4, Zhongguancun Dongsheng International Science Park, 1 North Yongtaizhuang Road, Haidian District, 100192 Beijing, China