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Fast Restriction Enzyme

  • FlyCut<sup>®</sup> KpnI
FlyCut<sup>®</sup> KpnI

FlyCut® KpnI


  • Product Introduction
  • Manual Download
  • References

Description

FlyCut® KpnI is expressed and purified from E.coli that carries the recombinant KpnI gene. The molecular weight is 25.9 kDa, with the recognition site at GGTAC^C. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.


Concentration


10,000 units/ml

Recognition Site


5’...GGTAC↓C...3’

3’...C↑CATGG...5’


Applications


Genomic DNA, plasmid DNA, PCR product

Unit Definition


One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.


Storage Buffer


20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

10×FlyCut® Buffer


500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

Storage

at -20℃ for two years


Kit Contents


Component

JK101-01

JK101-02

FlyCut® KpnI

2,000 units

2×2,000 units

10×FlyCut® Buffer

500 μl

1 ml

10×DNA Loading Buffer

1 ml

1 ml



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